A function that perform normalisation for alternative expression
crossSampleDoublets(sce, altExp_name = NULL, totalExp_threshold = 10)
| sce | A |
|---|---|
| altExp_name | Name of alternative expression that will be used to perform normalisation. If it is NULL, it will set to HTO. |
| totalExp_threshold | the threshold indicates for the HTO less than this threshold will be filtered from the analysis |
A SingleCellExperiment Object
data(CITEseq_example, package = "CiteFuse") sce_citeseq <- preprocessing(CITEseq_example) sce_citeseq <- normaliseExprs(sce = sce_citeseq, altExp_name = "HTO", transform = "log") sce_citeseq <- crossSampleDoublets(sce_citeseq)#> number of iterations= 20 #> number of iterations= 24 #> number of iterations= 46 #> number of iterations= 50